2 min read

ID: 1159830

Short Link: https://gregory-ms.com/articles/1159830/

Discovery Date: 30 December 2022, 17:30:37 UTC

Published Date: 2022-12-30 00:00:00

Source: BioMedCentral

Link: https://bmccomplementmedtherapies.biomedcentral.com/articles/10.1186/s12906-022-03825-5

Manual Selection: true

Machine Learning Gaussian Naive Bayes Model: false

Abstract

jats:titleAbstract</jats:title>jats:sec jats:titleBackground</jats:title> jats:pjats:italicCurcuma longa</jats:italic> has been used as spices, food preservative, coloring material, and traditional medicine. This plant also has long been used for a variety of diseases including dyslipidemia, stomach disorders, arthritis, and hepatic diseases. The aim of the present investigation was to examine the anti-neuroinflammatory effects of the 50% ethanolic extract of jats:italicC. longa</jats:italic> in lipopolysaccharide (LPS)-induced BV2 microglial cells.</jats:p> </jats:sec>jats:sec jats:titleMethods</jats:title> jats:pGriess reaction was employed to measure the production of nitric oxide (NO), and the levels of prostaglandin E2 (PGEjats:sub2</jats:sub>) and pro-inflammatory cytokines such as interleukin 1-beta (IL-1β), IL-6 and tumor necrosis factor-α (TNF-α) were determined by using profit ELISA kits. Western blotting was used to determine the expression of inducible nitric oxide synthase (iNOS), cyclooxygenase-2 (COX-2), nuclear factor kappa B (NF-κB), mitogen activated protein kinases (MAPKs), heme oxygenase-1 (HO-1) and nuclear factor erythroid-2-related factor 2 (Nrf2).</jats:p> </jats:sec>jats:sec jats:titleResults</jats:title> jats:pPre-treatment with CLE inhibited the overproduction and overexpression of pro-inflammatory mediators including NO, PGEjats:sub2</jats:sub>, iNOS, COX-2, and pro-inflammatory cytokines such as IL-1β, IL-6 and TNF-α in LPS-induced BV2 cells. In addition, CLE suppressed the activation of the NF-κB and three MAPK signaling pathways. Treatment with CLE induced HO-1 protein expression by activating Nrf2 pathway, and inhibiting the HO-1 expression reversed the anti-inflammatory effect of CLE.</jats:p> </jats:sec>jats:sec jats:titleConclusion</jats:title> jats:pCLE showed anti-neuroinflammatory effects against LPS-induced microglial cells activation through the inhibition of production and expression of pro-inflammatory mediators by negative regulation of the NF-κB and MAPK signaling pathways. These anti-neuroinflammatory effects of CLE were mediated by HO-1/Nrf2 signaling pathway. Taken together, the present study suggests a potent effect of CLE to prevent neuroinflammatory diseases. It is necessary to perform additional efficacy evaluation through in vivo experiments.</jats:p> </jats:sec>

Noun Phrases in Title

  • The ethanolic extract
  • Curcuma longa
  • Korea
  • anti-neuroinflammatory effects
  • nuclear transcription factor
  • erythroid-2-related factor
  • 2/heme oxygenase-1
  • pathway
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